1.

Shiraishi I. Basic and Comprehensive Outlines of Cardiovascular Embryology and Morphogenesis. Journal of Pediatric Cardiology and Cardiac Surgery 4: 63-74.

2.

Hoashi T, Ichikawa H, Nakata T, Shimada M, Ozawa H, Higashida A, Kurosaki K, Kanzaki S, Shiraishi I. Utility of a super-flexible three- dimensional printed heart model in congenital heart surgery. Interact Cardiovasc Thorac Surg. 2018 May 28. doi: 10.1093/icvts/ivy160

3.

Shiraishi I, Nishimura K, Sakaguchi H, Abe T, Kitano M, Kurosaki K, Kato H, Nakanishi T, Yamagishi H, Sagawa K, Ikeda Y, Morisaki T, Hoashi T, Kagisaki K, Ichikawa H. Acute rupture of chordae tendineae of the mitral valve in infants: a nationwide survey in Japan exploring a new syndrome. Circulation. 2014;130:1053-61.

4.

Shiraishi I, Kajiyama Y, Yamagishi M, Hamaoka K, Yagihara T. The applications of non-ECG-gated MSCT angiography in children with congenital heart disease. Int J Cardiol. 2012;156:309-14.

5.

Shiraishi I, Ichikawa H. Human heterotaxy syndrome – from molecular genetics to clinical features, management, and prognosis. Circ J. 2012;76(9):2066-75.

6.

Shiraishi I, Yamagishi M, Hamaoka K, Fukuzawa M, Yagihara T. Simulative operation on congenital heart disease using rubber-like urethane stereolithographic biomodels based on 3D datasets of multislice computed tomography. Eur J Cardiothorac Surg. 2010;37:302-6.

7.

Yamamoto Y, Shiraishi I, Dai P, Hamaoka K, Takamatsu T. Regulation of embryonic lung vascular development by vascular endothelial growth factor receptors, Flk-1 and Flt-1. Anat Rec. 2007;290:958-73.

8.

Sato H, Shiraishi I, Takamatsu T, Hamaoka K. Detection of TUNEL-positive cardiomyocytes and c-kit-positive progenitor cells in children with congenital heart disease. J Mol Cell Cardiol. 2007;43:254-61.

9.

Tsujita Y, Muraski J, Shiraishi I, Kato T, Kajstura J, Anversa P, Sussman MA. Nuclear targeting of Akt antagonizes cardiomyocyte hypertrophy. Proc Natl Acad Sci USA. 2006;103:11946-51.

10.

Shiraishi I, Kajiyama Y, Yamagishi M, Hamaoka K. Stereolithographic biomodeling of congenital heart disease by multislice computed tomography imaging. Circulation. 2006;113:e733-4.

11.

Shiraishi I, Melendez J, Ahn Y, Skavdahl M, Murphy E, Welch S, Schaefer E, Walsh K, Rosenzweig A, Torella D, Nurzynska D, Kajstura J, Leri A, Anversa P, Sussman MA. Nuclear targeting of Akt enhances kinase activity and survival of cardiomyocytes. Circ Res. 2004;94:884-91.

12.

Torella D, Rota M, Nurzynska D, Musso E, Monsen A, Shiraishi I, Zias E, Walsh K, Rosenzweig A, Sussman MA, Urbanek K, Nadal-Ginard B, Kajstura J, Anversa P, Leri A. Cardiac stem cell and myocyte aging, heart failure, and IGF-1 overexpression. Circ Res. 2004;94:514-24.

13.

Shiraishi I, Yamamoto Y, Ozawa S, Kawakita A, Toiyama K, Tanaka T, et al. Application of helical computed tomographic angiography with differential color imaging 3D reconstruction in the diagnosis of complicated congenital heart diseases. J Thorac Cardiovasc Surg. 2003;125:36-9.

14.

Shiraishi I, Kato Y, Todoroki H, Satoh H, Hamaoka K. Images in cardiovascular medicine. Differential color imaging technique of helical CT angiography in the diagnosis of total anomalous pulmonary venous drainage. Circulation. 2000;101:2017-8.

15.

Shiraishi I, Simpson DG, Carver W, Borg TK et al. Vinculin is an essential component for normal myofibrillar arrangement in fetal mouse cardiac myocytes. J Mol Cell Cardiol. 1997;29:2041-52.

16.

Shiraishi I, Takamatsu T, Price RL, Fujita S. Temporal and spatial patterns of phosphotyrosine during cardiac myofibrillogenesis of chicken embryo. Anat Embryol 1997;196:81-9.

17.

Shiraishi I, Takamatsu T, Fujita S. Three-dimensional observation with a confocal scanning laser microscope of fibronectin immunolabeling during cardiac looping in the chick embryo. Anat Embryol. 1995;191:183-9.

18.

Shiraishi I, Takamatsu T, Minamikawa T, Onouchi Z, Fujita S. Quantitative histological analysis of the human sinoatrial node during growth and aging. Circulation. 1992;85:2176-84.

19.

Shiraishi I, Takamatsu T, Fujita S. 3-D observation of N-cadherin expression during cardiac myofibrillogenesis of the chick embryo using a confocal laser scanning microscope. Anat Embryol. 1993;187:115-20.

20.

Shiraishi I, Takamatsu T, Minamikawa T, Fujita S. 3-D observation of actin filaments during cardiac myofibrinogenesis in chick embryo using a CLSM. Anat Embryol. 1992;185:401-8.

1

Shiraishi I. Left-Right Asymmetry and Human Heterotaxy Syndrome. In: Nakanishi T, Markwald RR, Baldwin HS, Keller BB, Srivastava D, Yamagishi H, editors. Etiology and Morphogenesis of Congenital Heart Disease: From Gene Function and Cellular Interaction to Morphology. Tokyo: Springer; 2016. Chapter 6.

2

Shiraishi I, Left-Right Asymmetry and Human Heterotaxy Syndrome. Etiology and Morphogenesis of Congenital Heart Disease. From Gene Function and Cellular Interaction to Morphology. Eds: Nakanishi T, Markwald RR, Baldwin HS. Springer, 2016, pp49-56.

3

Shiraishi I, Takamatsu T, Hamaoka K. Signal transduction during cardiac myofibrillogenesis and looping. In Cardiovascular Development and Congenital Heart Disease. Molecular and Genetic Mechanisms. Eds: Artman M, Benson w, Srivastava D, Nakazawa M. Blackwell Futura, 2005, pp17-19.

4

Shiraishi I, Takamatsu T, Onouchi Z, Borg TK. Cell-cell and cell-matrix adhesions during formation and arrangement of developing cardiac myofibrils. In etiology and Morphogenesis of Congenital Heart Disease: Twenty years of progress in genetics and developmenta;l biology. Eds: Clark EB, Nakazawa M, Taao A. Futura Publishing Co, Inc., 2000, pp175-180.

5

Shiraishi I, Takamatsu T, Onouchi Z, Fujita S. Three-dimensional observation of F-actin and expression of N-cadherin and fibronectin during cardiac looping of the chick embryo using CLSM. In Developmental Mechanisms of Heart Disease. Eds: Clark EB, Markward RR, Takao A. Futura Publising Co, Inc., 1995, pp465-476.

1

Yamagishi H. Shiraishi I (Eds.). Clinical Cardiac Embryology for Understanding Congenital Heart Disease, Second Edition (in Japanese). Tokyo: Medical View Co., Ltd., 2021.

2

Ichikawa H, Ouchi H, Kurosaki K, Shiraishi I (Eds). Fontan Circulation (in Japanese). Tokyo: Shindan To Chiryo Sha, 2020.

3

Japanese Society of Pediatric Cardiology and Cardiac Surgery (Chief editor: Shiraishi I). Pediatric and Developmental Cardiology and Cardiac Surgery (in Japanese). Tokyo: Shindan To Chiryo Sha, 2018.

4

Yamagishi H. Shiraishi I (Eds.). Clinical Cardiac Embryology for Understanding Congenital Heart Disease (in Japanese). Tokyo: Medical View Co., Ltd., 2007.

5

Hamaoka K, Shiraishi I (Eds.). Helical CT Angiography in Congenital Heart Disease. Tokyo: Medical-sense Co., Ltd., 2004.

1

Comprehensive research of intractable cardiovascular diseases that such as congenital heart disease that occurs in childhood, aiming at establishing a medical care system and improving survival rates, smooth transition of medical care, and prognosis in adulthood and beyond. Research Project on Overcoming Intractable Diseases FY2024-FY2026 by the Ministry of Health, Labour and Welfare. (Research director: Hideo Ohuchi)

2

Development and commercialization of the ‘ped UT-Heart’ cardiac simulator to support the determination of the optimal surgical procedure for the purpose of saving the lives of and improving the quality of life of patients with congenital heart disease. Japan Agency for Medical Research and Development (AMED), Medical-Engineering Innovation Promotion Project. FY2022-2025. (Representative: Isao Shiraishi)

3

Development and commercialization of a multi-scale/multi-physics heart simulator “ped UT-Heart” that allows for selection of an optimal treatment strategy, aiming at improving life-long QoL of pediatric patients with congenital heart disease. Program of Research on Development of Innovative Medical Devices and Systems FY2020–2022 by the Japan Agency for Medical Research and Development. (Research director: Isao Shiraishi)

4

Transcriptome and Metagenome Analysis of Patients with Acute Rupture of Chordae Tendineae of the Mitral Valve in Infants. Grant-in-Aid for Scientific Research (C) [FY2019–FY2021] by the Japan Society for the Promotion of Science. (Research director: Isao Shiraishi)

5

Comprehensive research of intractable cardiovascular disease such as congenital heart disease that occurs in childhood, aiming at establishing a medical care system and improving life-long QoL. Research Project on Overcoming Intractable Diseases FY2018–FY2020 by the Ministry of Health, Labour and Welfare. (Research director: Isao Shiraishi)

6

Development and commercialization of custom-made, super-soft, finely-detailed 3D heart models aiming at reducing surgical time in patients with congenital heart disease whose three-dimensional heart structure is extremely complex by using 3D heart models for diagnosis. Support Program on Collaborative Innovation Networks for Medical Device FY2017–2019 by the Japan Agency for Medical Research and Development. (Leader: Masatoshi Takeda; Subleader: Isao Shiraishi)

7

Research to establish a cardiac surgery training method and individualized medical care by creating super-soft, finely-detailed heart replicas. Program of Research on Development of Medical Devices FY2014–2016 by the Japan Agency for Medical Research and Development. (Research director: Isao Shiraishi)

8

Research to explore the ways to understand long-term prognosis of children with congenital heart disease into adulthood. Research Project on Overcoming Intractable Diseases FY2015–FY2017 by the Ministry of Health, Labour and Welfare. (Research director: Isao Shiraishi)

9

Research to establish a treatment strategy for acute rupture of chordae tendineae of the mitral valve in infants based on different pathogenesis. Health and Labor Sciences Research Grant FY2016–FY2017 by the Ministry of Health, Labour and Welfare. (Research director: Isao Shiraishi)

1

PTC-international patent：PTC/JP2010/061249, ”Method for producing resin modeled body and hollow structure body, and core”

2

PTC- international patent: PCT/JP2018/00371 ”VERIFICATION METHOD AND VERIFICATION SYSTEM FOR INTERAL ORGAN MODEL”